Please use this identifier to cite or link to this item: https://openscholar.ump.ac.za/handle/20.500.12714/981
Title: Morphological, molecular identification and genetic diversity assessment of three terrestrial isopods from the high-altitude region.
Authors: Hassan, Montaser M.
Albogami, Bander.
Mwabvu, Tarombera.
Hassan, Mohamed M.
Awad, Mohamed F.
Sabry, Ayman.
Ismail, Mohamed.
Farid, Mona A.
Elsharkawy, Mohsen Mohamed.
Taif University
Taif University
School of Biology and Environmental Sciences
Taif University
Taif University
Taif University
Menoufia University
Kafrelsheikh University
Kafrelsheikh University
Keywords: Armadillidium vulgare.;COI Gene.;Diversity.;Porcellio laevis.;Porcellionides pruinosus.
Issue Date: 2024
Publisher: Journal of Biobased Materials and Bioenergy
Abstract: Terrestrial specimens were collected from Ashshafa, a south-western highland area in Saudi Arabia. Three species, i.e., Porcellio laevis, Porcellionides pruinosus (Porcellionidae), and Armadillidium vulgare (Armadillidiidae), were identified in this study based on their morphological characteristics. Partial mitochondrial cytochrome C oxidase subunit I (COI) gene sequences were used for DNA barcoding and biodiversity assessments. A phylogenetic tree of 22 haplotypes from 35 specimens of the three isopod species was drawn from the most similar sequences obtained from BLAST with the associated accession numbers. The tree included two clades. The first clade included samples of P. laevis and P. pruinosus, whereas the second clade included samples of A. vulgare. Each identified species formed a distinct subclade within the main clade, along with similar sequences obtained from the NCBI database. The heat map of genetic distance among haplotypes shows the haplotype diversity (Hd) ranged from 0.590 to 0.933 (mean = 0.767) and total nucleotide diversity ( T) ranged from 0.001 to 0.089 (mean=0.049), with a similar trend observed for nucleotide diversity per site ( w) ranged from 0.001 to 0.80 (mean = 0.049). In contrast, synonymous nucleotide diversity ( s), mean=0.009, was low compared to nonsynonymous nucleotide diversity ( s), mean=0.060, across all species. In conclusion, the morphological identification of terrestrial isopods was confirmed using COI gene sequencing of mitochondrial DNA. These results will be helpful in developing a deeper isopod identification method.
Description: Published version
URI: https://openscholar.ump.ac.za/handle/20.500.12714/981
DOI: 10.1166/jbmb.2024.2405
Appears in Collections:Journal articles

Files in This Item:
File Description SizeFormat 
Morphological-molecular-identification-and-genetic-diversity-assessment-of-three-terrestrial-isopods-from-the-high-altitude-region..pdf
  Until 2025-07-04
published version824.36 kBAdobe PDFView/Open    Request a copy
Show full item record

Google ScholarTM

Check

Altmetric


Items in UMP Scholarship are protected by copyright, with all rights reserved, unless otherwise indicated.